Aim: To evaluate the effect of repeated bonding on the shear bond strength of orthodontic brackets using a cyanoacrylate adhesive. Material and Methods: Sixteen freshly extracted human molars were collected and stored in a solution of 0.1% (weight/volume) thymol. The teeth were cleaned, polished, and etched with a 35% phosphoric acid gel. The brackets were bonded with an adhesive that contains ethyl-cyanoacrylate. The teeth were sequentially bonded and debonded three times using the same orthodontic adhesive. All teeth were debonded within 1⁄2 hour after bonding to simulate the clinical condition in which a newly rebonded bracket is attached to the archwire. Results: The analysis of variance comparing the shear bond strength at the three debonding attempts indicated the presence of a significant difference between the three groups (F ratio = 8.277 and P = .001). Duncan posterior tests indicated that the shear bond strength at the initial debonding sequence was significantly stronger (mean = 5.7 ± 3.8 MPa) than the second (mean = 2.2 ± 2.6 MPa) and the third debonding sequences (mean = 2.1 ± 1.5 MPa). There were no significant differences between the last two debonding sequences. Conclusion: In general, the highest values for shear bond strength were obtained after the initial bonding. Rebonded teeth have significantly lower and inconsistent shear bond strengths. The changes in bond strengths may be related to the changes in the morphologic characteristics of the etched enamel surface as a result of the presence of adhesive remnants.