Home Subscription Services

World Journal of Orthodontics
WJO Home Page
About the Editor
Editorial Board
Reprints / Articles
Quintessence Publishing: Journals: ORTHODONTICS
The Art and Practice of Dentofacial Enhancement

Formerly World Journal of Orthodontics

Edited by
Rafi Romano, DMD, MSc (Editor-in-Chief)

ISSN 2160-2999 (print) / ISSN 2160-3006 (online)

Visit the ORTHODONTICS: The Art and Practice of Dentofacial Enhancement Facebook page

Winter 2005
Volume 6 , Issue 1

Share Abstract:

Bones, Teeth, and Genes: A Genomic Homage to Harry Sicher’s “Axial Movement of Teeth”

Sean Holliday, DDS, MS/Bernard Schneider, DDS, MS/Maria Therese Galang, DDS, MS/Tadayoshi Fukui, DDS, PhD/Akira Yamane, PhD/Xianghong Luan, MD/Thomas G.H. Diekwisch, DDS, PhD

Pages: 61–70
PMID: 15794043

Aim: The model of the unopposed rodent molar was used to study the morphologic and genetic mechanisms of tooth eruption. Methods: Left maxillary molar teeth of 12-day-old Swiss-Webster mice were extracted under anesthesia, and mandibular molars were allowed to supererupt. To trace areas of tissue remodeling and to determine areas of new tissue formation, mice were injected with fluorescent dyes, tetracycline, alizarin red, and calcein blue. Subsequent to sacrifice, mandibular tissue blocks were prepared for ultrathin ground sections, fluorescent microscopy, and von Kossa’s mineral detection procedure. A second set of specimens was prepared for RNA extraction and microarray analysis. Results: The data established significant eruption of first and second mandibular mouse molars 12 days after complete extraction of antagonists, exceeding the control side by 0.13 mm. Labeled tissue sections revealed significant amounts of new bone and cementum apposition on the unopposed side compared to the control side, as revealed by fluorescent markers and ultrathin ground sections. Microarray transcript level comparisons between the experimental and the control groups demonstrated significant (more than twofold) increase in gene expression of elastin and tenascin C extracellular matrix proteins; brevican, lumican, and biglycan proteoglycans; as well as fibroblast growth factor 9. Conclusion: In this study, the authors have established the unopposed mouse molar as a model to study tissue dynamics during the axial movement of teeth. The data indicated significant new formation of bone and cementum in tandem with increased expression of extracellular matrix-related genes. World J Orthod 2005;6:61–70.

Full Text PDF File | Order Article


Get Adobe Reader
Adobe Acrobat Reader is required to view PDF files.
This is a free program available from the Adobe web site.
Follow the download directions on the Adobe web site
to get your copy of Adobe Acrobat Reader.
  © 2017 Quintessence Publishing Co Inc

Home | Subscription Services | Books | Journals | Multimedia | Events | Blog
Terms of Use | Privacy Policy | About Us | Contact Us | Advertising | Help | Sitemap | Catalog