The aim of this study ws to evaluate the permeability of the disposable, nonsterile gloves used in dental practice. A visual inspection and a water-filling test were carried out on seven different brands of gloves. Only five of the seven makes analyzed showed a value of pinch marks statistically not different from 2% (the limit recommended by the American Society for Testing and Materials). Analysis of the gloves with holes revealed that only four makes showed a value statistically not different from 2%. Finally, only three of the seven makes analyzed had a number of defects statistically not different from 2%. It may therefore be stated that the average quality level currently available should be improved to guarantee a safer and more effective protection against oral pathogens in dental practice.