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Quintessence Publishing: Journals: QI
Quintessence International

Edited by Eli Eliav

ISSN 0033-6572 (print) • ISSN 1936-7163 (online)

Publication:
July/August 2001
Volume 32 , Issue 7

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Remineralization across the resin-dentin interface: In vivo evaluation with nanoindentation measurements, EDS, and SEM

Naotake Akimoto, DMD, PhD/Gen Yokoyama, DMD/Kaoru Ohmori, DMD, PhD/Shiro Suzuki, DDS, PhD/Atsushi Kohno, DDS, PhD/Charles F. Cox, DMD, FADI

Pages: 561-570
PMID: 11495568

Objective: The purpose of this study was to evaluate the in vivo remineralization of the possible non–resin infiltrated hybridoid layer between the hybrid layer and the subjacent dentin substrate using nanoindentation, energy dispersive x-ray spectroscopy microanalyses (EDS), and scanning electron microscopy (SEM) technologies. Method and materials: Twenty Class V cavities were placed in healthy adult monkey teeth. Each cavity was total etched with 37% phosphoric acid for 60 seconds, rinsed, and air dispersed, and SA-Primer was applied to the collagen layer. Cavities were divided into two groups: In group 1, Protect Liner (low-viscosity resin) and Clearfil AP-X (resin composite) were placed per manufacturer’s directions, and no bonding agent was placed on the acid-etched interface. In group 2, Clearfil Photobond (bonding agent) was applied, and Protect Liner and Clearfil AP-X were placed as in group 1. Teeth were observed at 7 days (control) and 6 months by nanoindentation, EDS, and SEM. Results: Six-month data showed an increased nanohardness in areas 5 µm adjacent to the demineralized or partially demineralized dentin interface. Following treatment with a conventional adhesive system on the acid-etched interface (group 2), there were increased nanohardness and calcium EDS measurements in the substrate just below the resin-dentin impregnated layer. Conclusion: Our 6-month in vivo nanoindentation and EDS data demonstrate that the non–resin infiltrated zone becomes remineralized following adhesive resin treatment.

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