Objective: To evaluate intraexaminer reliability in measurement of basic tooth color by use of a clinically applicable spectrophotometer. Method and Materials: Three examiners determined the L* (lightness), C* (chroma), and h* (hue) values and tooth color according to the Vita 3D-Master shade guide system (Vident), twice for 161 anterior teeth of 19 subjects by use of a spectrophotometer (Vita Easyshade 1, Vita Zahnfabrik). Between the 2 measurements on each tooth, the investigators had to completely remove the probe tip from the mouth. The reliability of the measurements was observed, and deviations between the 2 L*C*h* measurements, as well as DEab for the measurements, were calculated. Results: Exact agreement of chosen shade tab according to the 3D-Master shade guide was achieved in 48% of cases. Mean DL*, DC*, and Dh* for all examiners were 1.9 (SD 1.7), 1.3 (SD 1.3), and 0.5 degrees (SD 0.7 degrees), respectively. The maximum deviation was 9 for DL*, 7 for DC*, and 3 degrees for Dh*. Mean DEab for the measurements was 2.7 (SD 1.8, range 0 to 9.5). Conclusion: Intraexaminer reliability was usually acceptable, although a clinically relevant difference between the 2 measurements of an examiner was occasionally observed. This uncertainty should be borne in mind in clinical routine and in studies reporting changes in color, eg, studies of tooth bleaching. (Quintessence Int 2009;40:421–426)