Home Subscription Services
 
   

 
Quintessence International
QI Home Page
About the Editor
Editorial Board
Accepted Manuscripts
Submit
Author Guidelines
Submission Form
Reprints / Articles
Permissions
Advertising
MEDLINE Search
 
 
 
 
 
FacebookTwitterYouTube
Quintessence Publishing: Journals: QI
Quintessence International

Edited by Eli Eliav

ISSN 0033-6572 (print) • ISSN 1936-7163 (online)

Publication:
June 2008
Volume 39 , Issue 6

Back
Share Abstract:

Cytotoxicity of recent dentin bonding agents on mouse fibroblast cells

Sias R. Grobler, DSc, PhD/Annette Oliver, MSc/Desi Moodley, PhD/Theunis J. Van Wyk Kotze, DSc

Pages: 511–516
PMID: 19057749

Objectives: To compare the cytotoxicity of the polymerized bonding agents Scotchbond 1 (3M ESPE), Prime & Bond NT (Dentsply DeTrey), Xeno III (Dentsply DeTrey), and Clearfil Protect Bond (primer and bond parts; Kuraray) on mouse fibroblast cells. Xeno III was also tested through thin dentin disks. Method and Materials: Near-confluent 3T3 cells were exposed to Dulbecco modified eagle’s medium extractions from the above-mentioned agents, and the cell viability (survival rate) was measured using the standard MTT assay and related to the nonexposed controls. Results: All bonding agents were found to be cytotoxic toward the 3T3 cells. Scotchbond 1 (59% survival rate) and Prime & Bond NT (62% survival rate) were not statistically different (Kruskal-Wallis test, P > .05). However, the survival rates of Xeno III (25% through membrane and dentin disks) and Clearfil Protect Bond (35%) were significantly lower than those of the other 2 bonding agents, with Xeno III significantly the most toxic (Kruskal-Wallis test, P < .05). Of the 2 parts from Clearfil Protect Bond, the bond part was the least toxic (91% survival rate), but the primer part was very toxic (30% survival rate). Conclusion: In an in vitro culture, all 4 dentin bonding agents were cytotoxic. Xeno III was the most toxic, even through dentin disks. It seems that the cytotoxicities depend on the compositions of the materials tested. The most toxic part of Clearfil Protect Bond was the primer, which contains the antibacterial pyridinium molecule. (Quintessence Int 2008;39:511–516)

Key words: bonding agents, cytotoxicity, mouse fibroblast

Full Text PDF File | Order Article

 

Get Adobe Reader
Adobe Acrobat Reader is required to view PDF files. This is a free program available from the Adobe web site.
Follow the download directions on the Adobe web site to get your copy of Adobe Acrobat Reader.
  © 2014 Quintessence Publishing Co Inc
 

Home | Subscription Services | Books | Journals | Multimedia | Events | Blog
Terms of Use | Privacy Policy | About Us | Contact Us | Advertising | Help | Sitemap | Catalog