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Quintessence Publishing: Journals: QI
Quintessence International

Edited by Eli Eliav

ISSN 0033-6572 (print) • ISSN 1936-7163 (online)

June 2007
Volume 38 , Issue 6

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In vitro antimicrobial activity of peroxide-based bleaching agents

Marcelo Henrique Napimoga, DDS, MS, PhD / Rogerio de Oliveira, DDS, MS / Andre Figueiredo Reis, DDS, MS, PhD / Reginaldo Bruno Gonçalves, DDS, MS, PhD / Marcelo Giannini, DDS, MS, PhD

PMID: 17625621

Antibacterial activity of 4 commercial bleaching agents (Day White, Colgate Platinum, Whiteness 10% and 16%) on 6 oral pathogens (Streptococcus mutans, Streptococcus sobrinus, Streptococcus sanguinis, Candida albicans, Lactobacillus casei, and Lactobacillus acidophilus) and Staphylococcus aureus were evaluated. A chlorhexidine solution was used as a positive control, while distilled water was the negative control. Bleaching agents and control materials were inserted in sterilized stainless-steel cylinders that were positioned under inoculated agar plate (n = 4). After incubation according to the appropriate period of time for each microorganism, the inhibition zones were measured. Data were analyzed by 2-way analysis of variance and Tukey test (a = 0.05). All bleaching agents and the chlorhexidine solution produced antibacterial inhibition zones. Antimicrobial activity was dependent on peroxide-based bleaching agents. For most microorganisms evaluated, bleaching agents produced inhibition zones similar to or larger than that observed for chlorhexidine. C albicans, L casei, and L acidophilus were the most resistant microorganisms. (Quintessence Int 2007;38:528.e329–333)

Key words: antimicrobial activity. bleaching agent, carbamide peroxide, oral microorganism

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