This case report study examined the effect of adjunctive use of Emdogain in the treatment of intrabony periodontal defects. Seventy-two consecutively treated defects in sixty-one patients were included. Efficacy of treatment was evaluated at 12 months by assessment of probing depth reduction, probing attachment level gain, and radiographic bone gain from standardized radiographs. Initial pocket depth averaged 8.3 mm, and the mean probing attachment level was 10.0 mm. The mean radiographic defect depth was 5.3 mm. At 12 months, mean pocket depth reduction was 4.7 mm and mean probing attachment level gain was 4.2 mm. Radiographic bone level gain averaged 3.1 mm and defect fill averaged 70%. The only variables significantly affecting radiographic bone gain were bleeding on probing and smoking. It was concluded that Emdogain treatment of one- and two-walled intrabony defects in a periodontal practice will result in a clinically significant gain of probing attachment level and radiographic bone that is similar to that reported in controlled clinical trials.