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Volume 30 , Issue 1
January/February 2010

Pages 83-87


Anorganic Bovine Bone (Bio-Oss) Regulates miRNA of Osteoblast-like Cells

Annalisa Palmieri, PhD/Furio Pezzetti, PhD/Giorgio Brunelli, MD/Marcella Martinelli, PhD/Lorenzo Lo Muzio, MD/Antonio Scarano, DDS/Luca Scapoli, PhD/Marzia Arlotti, PhD/Laura Guerzoni, PhD/Francesco Carinci, MD


PMID: 20224834
DOI: 10.11607/prd.00.0901

Bio-Oss (Geistlich) is composed of anorganic bovine bone and has been widely used in several bone regeneration procedures during oral surgery. However, how this biomaterial enhances osteoblast activity to promote bone formation is not completely understood. MicroRNAs (miRNAs) represent a class of small, functional, noncoding RNAs of 19 to 23 nucleotides that regulate the transcription of messenger RNAs (mRNAs) in proteins. In this study, the miRNA microarray technique was used to investigate translation regulation in an osteoblast-like cell line (MG63) exposed to Bio-Oss. Nine up-regulated miRNAs (mir-423, mir-492, mir-191, mir-23a, mir-377, mir-494, mir-214, mir-193b, mir-320) and 4 down-regulated miRNAs (mir-27a, mir-24, mir-188, let-7c) were identified. Because each miRNA regulates 100 mRNAs, only mRNAs related to bone formation were analyzed. The vast majority of detected mRNAs are down-regulated, including some homeobox genes (genes that regulate the morphogenesis of an entire segment of the body), such as noggin and EN1. An indirect positive effect was demonstrated on bone morphogenetic protein-4. To the authors’ knowledge, the data reported here are the first on translation regulation in osteoblasts exposed to Bio-Oss. This study may be relevant in better understanding the molecular mechanism of bone regeneration and used as a potential tool for analyzing the combined use of cytokines.(Int J Periodontics Restorative Dent 2010;30:83–87.)


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