LOGIN
 
Share Page:
Back

Volume 26 , Issue 3
May/June 2011

Pages 509519


Attachment and Proliferation of Human Osteoblast-like Cells on Guided Bone Regeneration (GBR) Membranes in the Absence or Presence of Nicotine: An In Vitro Study

Konstantinos A. Papaioannou, DDS, MS/Cleopatra E. Markopoulou, DDS, DrDent/Vassiliki Gioni, MSc/Anastasios A. Mamalis, BSc, DDS, MBA, MSc, PhD/Helen N. Vavouraki, BSPharm, PhD/Dimitris Kletsas, PhD/Ioannis A. Vrotsos, DDS, Dr Dent


PMID: 21691597

Purpose: To compare in vitro the attachment and proliferation of human osteoblast-like cells (MG63) on tissue culture plates and guided bone regeneration (GBR) membranes in the absence or presence of nicotine. Materials and Methods: Membrane samples were fixed to wells and the cell number (CN) was counted after 24 hours (attachment assay) or 5 days (proliferation assay). The ratio of cell count (RCC) (CN at 5 days/CN at 24 hours) was calculated. The study had three parts: First, five different resorbable GBR membranes were compared (Resolut Adapt LT [RALT], Biocollagen [BC], Bio-Gide [BG], OsseoGuard [OG], and Demokritos Human Tissue Bank [DEM]). Next, cells were cultured on tissue culture plates with five different concentrations of nicotine. Finally, cells were cultured on the membrane that had demonstrated the highest RCC and CN in part 1 with four different concentrations of nicotine. Results: At 24 hours, BG showed the highest CN and OG showed the lowest CN. At 5 days, BG showed the highest CN. The order of RCC was BG > OG > DEM > RALT ~ BC. At 24 hours, lower nicotine concentrations (0.3 and 3 g/mL) showed higher CNs versus the control, whereas CNs for high nicotine concentrations (30 and 300 g/mL) were lower than for the control. CN at 5 days and RCC were lowest with 300 g/mL nicotine. At 24 hours and 5 days, all differences among wells with membrane were statistically insignificant. Nevertheless, CN at 5 days and RCC were highest with the lowest nicotine concentration (3 g/mL) and lowest with high concentrations. Conclusions: Membrane materials influence attachment and proliferation of bone cells and, therefore, could affect the outcomes of GBR. On both tissue culture plates and membranes, there is a tendency toward a biphasic effect of nicotine, with stimulatory effects at low concentrations and inhibitory effects at high concentrations. Int J Oral Maxillofac Implants 2011;26:509519

Key words: cell attachment, cell proliferation, guided bone regeneration, membrane, nicotine, osteoblast


Full Text PDF File | Order Article

 

 
Get Adobe Reader
Adobe Acrobat Reader is required to view PDF files. This is a free program available from the Adobe web site.
Follow the download directions on the Adobe web site to get your copy of Adobe Acrobat Reader.

 

© 2017 Quintessence Publishing Co, Inc JOMI Home
Current Issue
Ahead of Print
Archive
Author Guidelines
About
Accepted Manuscripts
Submission Form
Submit
Reprints
Permission
Advertising
Quintessence Home
Terms of Use
Privacy Policy
About Us
Contact Us
Help