• Submission Form (PDF)
• Author Guidelines (PDF)
• Submit

• Accepted Manuscripts

Back

Volume 8 , Issue 3
May/June 1993
Pages 264-272

Binding of Murine Osteoblastic Cells to Titanium Disks and Collagen I Gels: Implications for Alternative Interpretations of Osseointegration

Lyndon F. Cooper, DDS, PhD / Beverly Handelman / Sandra M. McCormack /Albert D. Guckes, DDS, MSD

PMID: 8225461

Using 3H-thymidine-labeled MC3T3-E1 osteoblastic cells, the number of osteoblasts bound to titanium surfaces after various surface treatments and incubation periods was directly measured. MC3T3-E1 cell binding to titanium surfaces was saturable at a low level (approximately 10,000 cells/cm2). Although treatment of these surfaces with fibronectin, keratin sulfate, and the fibronectin-derived peptide GRGDS (glycine-arginine-glycine-glutamate-serine) increases cellular binding by 29% to 31%, the relative binding to titanium was 5 to 10 times lower than binding to collagen I gels. A collagen I matrix competed with the commercially pure titanium surfaces for cell binding from solution, suggesting that direct binding of osteoblasts to titanium surfaces present within an organic matrix may not be favored. The significance of immediate and direct bone cell attachment to titanium surfaces for osseointegration should be reevaluated. (INT J ORAL MAXILLOFAC IMPLANTS 1993;8:264-272.)

Key words: cell attachment, osseointegration, titanium

Full Text PDF File | Order Article

 

 

Adobe Acrobat Reader is required to view PDF files. This is a free program available from the Adobe web site.
Follow the download directions on the Adobe web site to get your copy of Adobe Acrobat Reader.