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Volume 1 , Issue 1
Summer 1986

Pages 31-37

An Improved Method for Preparing Histological Sections of Metallic Implants

C. Maniatopoulos

PMID: 3527956

An improved method for preparing ground tissue-implant sections of good quality for light microscopy is presented. The specimen is embedded in methacrylate and cut using a diamond wafering saw. The cut surface is polished and stained with Stevenel’s blue and a glass microscope slide is glued to the surface of the specimen using epoxy resin adhesive. A section is prepared, reduced to 30 to 50 μm using petrographic grinding techniques and protected by a glass coverslip. This method provides high quality sections in which fine tissue architecture, cellular detail, and the tissue-implant interface are preserved.

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