LOGIN
 
Share Page:
Back

Volume 22 , Issue 5
September/October 2007

Pages 701–709


Effects of Phosphated Titanium and Enamel Matrix Derivatives on Osteoblast Behavior In Vitro

J. Anthony Dacy, DDS, MS/Robert Spears, PhD/William W. Hallmon, DMD, MS/David G. Kerns, DMD, MS/Francisco Rivera-Hidalgo, DMD, MS/Zoran S. Minevski, PhD/Carl J. Nelson, ME/Lynne A. Opperman, PhD


PMID: 17974103

Purpose: The purpose of this study was to evaluate the effects of phosphated titanium and enamel matrix derivatives (EMD) on osteoblast function. Materials and Methods: Primary rat osteoblasts were cultured on disks of either phosphated or nonphosphated titanium. In half of the samples 180 µg of EMD was immediately added. The medium was changed every 2 days for 28 days and then analyzed using transforming growth factor-b1 (TGF-b1) and interleukin-1b (IL-1b) enzyme-linked immunosorbent assays (ELISAs). Scanning electron microscopy and light microscopy were used to evaluate nodule formation and mineralization. Results: Microscopic evaluation revealed no differences in osteoblast attachment between the 4 groups. Osteoblast nodule formation was observed in all groups. In the absence of mineralizing media, nodules on the nonphosphated titanium samples showed no evidence of mineralization. All nodules on the phosphated titanium had evidence of mineralization. ELISA revealed no significant differences in IL-1b production between any of the groups. The EMD-treated osteoblasts produced significantly more TGF-b1 than non–EMD-treated cells for up to 8 days, and osteoblasts on phosphated titanium produced significantly more TGF-b1 at 8 days. Discussion and Conclusion: Osteoblast attachment appeared unaffected by surface treatment. EMD initiated early TGF-b1 production, but production decreased to control levels within 10 days. Phosphated titanium increased TGF-b1 production at 8 days and induced nodule mineralization even in the absence of mineralizing medium. Int J Oral Maxillofac Implants 2007;22:701–709

Key words: enamel matrix derivatives, interleukin-1b, osteoblasts, phosphate titanium, transforming growth factor-b1


Full Text PDF File | Order Article

 

 
Get Adobe Reader
Adobe Acrobat Reader is required to view PDF files. This is a free program available from the Adobe web site.
Follow the download directions on the Adobe web site to get your copy of Adobe Acrobat Reader.

 

© 2014 Quintessence Publishing Co, Inc JOMI Home
Current Issue
Ahead of Print
Archive
Author Guidelines
About
Accepted Manuscripts
Submission Form
Submit
Reprints
Permission
Advertising
Quintessence Home
Terms of Use
Privacy Policy
About Us
Contact Us
Help