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Volume 20 , Issue 1
January/February 2005

Pages 118123

Comparison of the Platelet Concentrate Collection System with the Plasma-Rich-in-Growth-Factors Kit to Produce Platelet-Rich Plasma: A Technical Report

Gernot Weibrich, MD, DDS, PhD/Wilfried K. G. Kleis, MD/Walter E. Hitzler, MD/Gerd Hafner, MD

PMID: 15747683

Purpose: The aim of this study was to compare a new method for the production of platelet-rich plasma (PRP), the plasma-rich-in-growth-factors kit (PRGF kit; G.A.C. Medicale San Antonio, Vitoria, Spain), with an established method, the Platelet Concentrate Collection System (PCCS; 3i/Implant Innovations, Palm Beach Gardens, FL) with respect to resulting cellular and growth factor contents. Materials and Methods: Whole blood was drawn from 51 healthy donors (20 men, 31 women) aged 19 to 59 years (mean SD 35.12 9.65 years), and PRP was prepared by both methods. Results: Platelet counts differed significantly (signed rank test, P < .001 for all) between the donor blood (274,200 54,050/L), the PCCS PRP preparation (1,641,800 426,820/L), and the PRGF kit PRP preparation (513,630 139,470/L). The PCCS concentrated leukocytes (whole blood, 6,992 2,011/L; PCCS PRP, 14,153 7,577/L), while the PRGF kit produced a leukocyte-poor PRP (65 108/L). Higher concentrations of transforming growth factor ß1 (TGF-ß1) and platelet-derived growth factor AB (PDGF-AB) were found in the PCCS PRP (TGF-ß1, 290 95 ng/mL; PDGF-AB, 157 62 ng/mL) than in the Anitua PRGF kit PRP (TGF-ß1, 73 26 ng/mL; PDGF-AB, 47 21 ng/mL). Statistical analysis showed significant differences (P < .001 for TGF-ß1 and P < .01 for PDGF-AB). Discussion: The results of this study and some data in the literature indicate that the content of growth factors in PRP can vary tremendously, depending on the system used for the preparation of PRP. Conclusion: PCCS collects more platelets and leukocytes than the PRGF kit. This results in significantly higher growth factor levels. Further in vivo studies are needed to determine whether this results in a clinically different biologic effect. Int J Oral Maxillofac Implants 2005;20:118123

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