LOGIN
 
Share Page:
Back

Volume 31 , Issue 1
January/February 2016

Pages 232239


Effect of Cell Seeding Conditions on the Efficiency of In Vivo Bone Formation

Akiko Hori/Hideki Agata, DDS, PhD/Megumi Takaoka/Arinobu Tojo, MD, PhD/Hideaki Kagami, DDS, PhD


PMID: 26800183
DOI: 10.11607/jomi.4729

Purpose: To optimize methods for seeding cells on granular-type beta-tricalcium phosphate (β-TCP). Materials and Methods: Bone marrow stromal cells were obtained from rat long bones and cultured in flasks with Minimum Essential Medium, Alpha Modification (αMEM) supplemented with 10% fetal bovine serum (FBS), dexamethasone, ascorbic acid, β -glycerophosphate, and antibiotics. The influence of differential cell seeding densities and dynamic cell seeding conditions (rotation) was investigated using different sizes of β -TCP granules and a subcutaneous implantation model. Results: Higher cell seeding densities contributed to efficient in vivo bone formation. The rotational seeding did not affect the efficiency but contributed to the uniformity. Although the granule size did not affect the efficiency under the conditions used in this study, large granules showed more uniform distribution of bone regeneration, while small granules showed nonuniform but dense bone formation. Mixtures of relatively large and small granules may be beneficial for both uniform and efficient bone regeneration. Conclusion: These findings may contribute to stable bone tissue engineering with bone marrow stromal cells and β -TCP granules as a scaffold.


Full Text PDF File | Order Article

 

 
Get Adobe Reader
Adobe Acrobat Reader is required to view PDF files. This is a free program available from the Adobe web site.
Follow the download directions on the Adobe web site to get your copy of Adobe Acrobat Reader.

 

© 2017 Quintessence Publishing Co, Inc JOMI Home
Current Issue
Ahead of Print
Archive
Author Guidelines
About
Accepted Manuscripts
Submission Form
Submit
Reprints
Permission
Advertising
Quintessence Home
Terms of Use
Privacy Policy
About Us
Contact Us
Help