LOGIN
 
Share Page:
Back

Volume 14 , Issue 6
November/December 1999

Pages 785–797


The Effects of the Coupling of Titanium Implants and Dissimilar Metal Abutments on Osteoblast Differentiation In Vitro

James C. Taylor, DMD, Gail I. Anderson, BVSc, MSc, PhD, Elliott J. Sutow, PhD, MEd, Carl F. Driscoll, DMD, Douglas C. Mackey, BSc, BEd, DDS


PMID: 10612915

This study evaluated the effect of titanium endosseous dental implants coupled to dissimilar materials on the capacity of preosteoblasts in bone marrow culture to differentiate, to form alkaline phosphatase–positive colonies, and to mineralize. Ten UCLA abutments were cast in each of 4 alloys: Type III gold, ceramometal gold, commercially pure grade I titanium, and titanium-aluminum-vanadium (Ti-6AI-4V); 10 ceramic abutments and 30 sterile Brånemark System implants were also used. Five abutments of each material and 5 implants were incubated individually in rat bone marrow culture, as were 5 of each abutment attached to an implant; bone marrow cultures not containing test samples were used as controls. Following 17 days of culture, the solution potentials of individual abutments (except ceramic), the implant, and the implant-abutment couples were measured in the test medium. One dish of each group of 5 was then stained for bone nodule mineralization; the remainder were quantified by area for alkaline phosphatase staining. Statistical analysis of measured in vitro potentials showed that the uncoupled samples formed 2 groups, and coupled samples formed 3 groups. Analysis of variance for alkaline phosphatase–positive area values showed no significant differences between coupled or uncoupled groups and the control. Normal cell differentiation and morphology, as well as a lack of zones of inhibition, were observed. Bone nodule mineralization was evident in all groups. It was concluded that the presence of these commonly used implant abutment biomaterials coupled to titanium endosseous dental implants had no adverse effects on the in vitro capacity of preosteoblasts in marrow to differentiate and to form mineralized bone nodules, despite measured differences in solution potentials. (Int J Oral Maxillofac Implants 1999;14:785–797) Key words: biocompatible materials, cell culture, ceramic, corrosion, dental alloys, dental implants, electrochemistry, gold alloys, intraoral electrogalvanism, titanium


Full Text PDF File | Order Article

 

 
Get Adobe Reader
Adobe Acrobat Reader is required to view PDF files. This is a free program available from the Adobe web site.
Follow the download directions on the Adobe web site to get your copy of Adobe Acrobat Reader.

 

© 2014 Quintessence Publishing Co, Inc JOMI Home
Current Issue
Ahead of Print
Archive
Author Guidelines
About
Accepted Manuscripts
Submission Form
Submit
Reprints
Permission
Advertising
Quintessence Home
Terms of Use
Privacy Policy
About Us
Contact Us
Help