LOGIN
 
Share Page:
Back

Volume 28 , Issue 5
September/October 2013

Pages e283e289


Effects of Self-Assembling Peptide Hydrogel Scaffold on Bone Regeneration with Recombinant Human Bone Morphogenetic Protein-2


Masayuki Ikeno, DDS/Hideharu Hibi, DDS, PhD/Kazuhiko Kinoshita, DDS, PhD/Hisashi Hattori, DDS, PhD/Minoru Ueda, DDS, PhD


PMID: 24066345
DOI: 10.11607/jomi.te09

Purpose: The objective of this pilot study was to histologically evaluate bone regeneration using a self-assembling peptide hydrogel scaffold with recombinant human bone morphogenetic protein-2 (rhBMP-2) in a rabbit calvaria model. Materials and Methods: Five adult New Zealand White rabbits were used for the study. Each received four titanium cylinders, which were placed into perforated slits made in the outer cortical bone of the calvaria. The cylinders were filled with the following test materials: (1) unfilled control; (2) rhBMP-2; (3) PuraMatrix (PM), a synthetic self-assembling peptide (RADA16-I) consisting of a 16-amino acid sequence and with a three-dimensional structure; and (4) PM/rhBMP-2. Each cylinder was covered with a titanium lid. After 8 weeks, the animals were sacrificed, and ground sections were obtained for histomorphometric analysis. Results: Histomorphometric analysis showed that regenerated tissue in the cylinder with PM/rhBMP-2 was significantly increased compared to the empty control. The mean area values of regenerated tissue in the cylinders were 35.80% 10.35% (control), 47.94% 5.65% (rhBMP-2), 48.94% 11.33% (PM), and 58.06% 14.84% (PM/rhBMP-2). The mean area values of newly formed bone in the cylinders were 9.39% 4.34% (control), 14.03% 2.25% (rhBMP-2), 13.99% 2.15% (PM), and 16.61% 3.79% (PM/rhBMP-2). Neither rhBMP-2 nor PM alone significantly enhanced bone regeneration compared to the empty control cylinder. Conclusions: PM with rhBMP-2 significantly enhanced bone regeneration on the bone augmentation model in a rabbit. PM promises to be a useful alternative synthetic material as a carrier for rhBMP-2 for bone regeneration.


Full Text PDF File | Order Article

 

 
Get Adobe Reader
Adobe Acrobat Reader is required to view PDF files. This is a free program available from the Adobe web site.
Follow the download directions on the Adobe web site to get your copy of Adobe Acrobat Reader.

 

© 2014 Quintessence Publishing Co, Inc JOMI Home
Current Issue
Ahead of Print
Archive
Author Guidelines
About
Accepted Manuscripts
Submission Form
Submit
Reprints
Permission
Advertising
Quintessence Home
Terms of Use
Privacy Policy
About Us
Contact Us
Help