Share Page:

Volume 27 , Issue 6
November/December 2012

Pages 1340-1350

Comparison of Three Inoculation Methods for Bone Tissue Engineering

Gu Cheng, MD/Xi Chen, MD/Zhi Li, DDS, PhD/Hui Lu, MD/Ongodia Davide, MD/Zubing Li, DDS, PhD

PMID: 23189283

Purpose: The purpose of this study was to compare the effectiveness of three methods of cell inoculation on cell growth and bone formation: inoculation of seed cells into the scaffolds from two sides (two-side inoculation method), inoculation of seed cells from one side (single-side inoculation method), and inoculation of a compound of seed cells and type 1 collagen gel from one side (type 1 collagen inoculation method). Materials and Methods: Bone marrow stromal cells were isolated from 1-month-old male New Zealand rabbits and implanted into three-dimensional chitosan/beta-tricalcium phosphate scaffolds using the three different methods. Cultures were analyzed by various methods. Results: The type 1 collagen group expressed the best uniformity of cell distribution among all the three methods during a 1-week culture period, and the two-side group expressed the best uniformity during a 2- to 3-week culture period. The number of inoculated cells in the type 1 collagen group outpaced that of the other groups. With respect to the depth of penetration of the inoculated cells, the cells of the type 1 collagen group were concentrated on the surface of the scaffold and formed multiple layers, whereas the two-side group accounted for the deepest cell penetration. Conclusion: The two-side inoculation method improves the number and distribution of seed cells in vitro and enhances the quality and rate of bone formation in vivo. This method is the most suitable seed method for bone tissue engineering. Int J Oral Maxillofac Implants 2012;27:13401350

Key words: bone marrow stromal cells, distribution, inoculation, scaffold

Full Text PDF File | Order Article


Get Adobe Reader
Adobe Acrobat Reader is required to view PDF files. This is a free program available from the Adobe web site.
Follow the download directions on the Adobe web site to get your copy of Adobe Acrobat Reader.


© 2017 Quintessence Publishing Co, Inc JOMI Home
Current Issue
Ahead of Print
Author Guidelines
Accepted Manuscripts
Submission Form
Quintessence Home
Terms of Use
Privacy Policy
About Us
Contact Us