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Quintessence Publishing: Journals: OHPD


Oral Health & Preventive Dentistry

Edited by Jean-Francois Roulet, Poul Erik Petersen, Anton Sculean

Official journal of the World Congress of Minimally Invasive Dentistry

ISSN (print) 1602-1622 • ISSN (online) 1757-9996


Spring 2014
Volume 12 , Issue 1

Pages: 55-59
DOI: 10.3290/j.ohpd.a31225
Share Abstract:

Production of Extracellular Matrix Proteins by Human Pulp Fibroblasts in Contact with Papacárie and Carisolv

Bussadori, Sandra Kalil / Amancio, Olga Maria Silverio / Martins, Manoela Domingues / Guedes, Carolina Cardoso / Alfaya, Thays Almeida / Santos, Elaine Marcílio / França, Cristiane Miranda

Purpose: To evaluate the effect of two products for the chemomecanical removal of carious tissue (Papacárie and Carisolv) on human dental pulp fibroblasts and the synthesis of extracellular matrix proteins.
Materials and Methods: Fibroblasts were divided into three groups: group 1 (control), group 2 (Papacárie) and group 3 (Carisolv). Collagen I, III , fibronectin and osteonectin were analysed by immunofluorescence and compared among the groups.
Results: The groups exhibited similar immunolabeling for vimentin, type I collagen and fibronectin, but were negative for type III collagen. Osteonectin staining was strongly positive in the cells treated with Papacárie and Carisolv and weakly positive in the control group.
Conclusion: The findings of the present study showed that Papacárie and Carisolv are not cytotoxic to pulp fibroblast cells. Moreover, these products stimulate fibroblasts to produce osteonectin, likely leading to the formation of dentin matrix. These findings confirm the safe, beneficial use of both gels in minimally invasive techniques.

Keywords: dental pulp, extracellular matrix proteins, osteonectin, type I collagen, type III collagen

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