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Volume 32 , Issue 1
Winter 2018

Pages 75–83

Tumor Necrosis Factor Alpha Signaling in Trigeminal Ganglion Contributes to Mechanical Hypersensitivity in Masseter Muscle During Temporomandibular Joint Inflammation

Reio Ito, DDS/Masamichi Shinoda, DDS, PhD/Kuniya Honda, DDS, PhD/Kentaro Urata, DDS, PhD/Jun Lee, DDS, PhD/Mitsuru Maruno, DDS/Kumi Soma, DDS/Shinji Okada, DDS/Nobuhito Gionhaku, DDS, PhD/Koichi Iwata, DDS, PhD

PMID: 29145524
DOI: 10.11607/ofph.1854

Aims: To determine the involvement of tumor necrosis factor alpha (TNFα) signaling in the trigeminal ganglion (TG) in the mechanical hypersensitivity of the masseter muscle during temporomandibular joint (TMJ) inflammation. Methods: A total of 55 male Sprague-Dawley rats were used. Following injection of Complete Freund’s Adjuvant into the TMJ, the mechanical sensitivities of the masseter muscle and the overlying facial skin were measured. Satellite glial cell (SGC) activation and TNFα expression in the TG were investigated immunohistochemically, and the effects of their inhibition on the mechanical hypersensitivity of the masseter muscle were also examined. Student t test or two-way repeated-measures analysis of variance followed by Bonferroni multiple comparisons test were used for statistical analyses. P < .05 was considered to reflect statistical significance. Results: Mechanical allodynia in the masseter muscle was induced without any inflammatory cell infiltration in the muscle after TMJ inflammation. SGC activation and an increased number of TNFα-immunoreactive cells were induced in the TG following TMJ inflammation. Intra-TG administration of an inhibitor of SGC activity or of TNFα-neutralizing antibody depressed both the increased number of TG cells encircled by activated SGCs and the mechanical hypersensitivity of the masseter following TMJ inflammation. Conclusion: These findings suggest that persistent masseter hypersensitivity associated with TMJ inflammation was mediated by SGC–TG neuron interactions via TNFα signaling in the TG.

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