Purpose: It was hypothesized that mesenchymal stem cell (MSC)–derived growth factor applied by iontophoretic transdermal delivery might improve ultraviolet beam (UVB)–induced photoaged skin. This experimental study was performed to confirm this hypothesis. Materials and Methods: Photoaged skin was induced in nude rat after an 8-week regimen of UVB irradiation, and an MSC-conditioned medium (MSC-CM) was conducted by an iontophoretic transdermal transport system. The effect of iontophoresis with MSC-CM was evaluated by replica method and measurement of the hyaluronic acid level in the dermis after treatment. Results: MSC-CM conducted by iontophoresis significantly reduced all parameters for wrinkles and increased hyaluronic acid in the dermis. Conclusion: MSC-CM application by iontophoresis can be considered as a supplemental treatment to the methods for skin rejuvenation. ORAL CRANIOFAC TISSUE ENG 2012;2:115–121

Key words: growth factor, iontophoresis, skin rejuvenation, stem cell