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Quintessence Publishing: Journals: OBM


Oral Biosciences & Medicine

Edited by Birgitte Nauntofte, Jesper Reibel. Peter A. Reichart, Jim Sciubba, and Joanna M. Zakrzewska

Official publication of the European Society for Oral Laser Applications

ISSN 1742-3287

Oral Biosciences & Medicine

Year 2005
Volume 2 , Issue 1

Pages: 15 - 19

Periodontal Ligament Fibroblast Interleukin-6 Release after Exposure to Nicotine

Olson, Byron L./Warner, Ned A./Gregory, Richard L./McDonald, James/Zapata, Carlos A.

Purpose: To determine the influence of nicotine upon the release of interleukin-6 (IL-6) from human periodontal ligament fibroblasts (HPLF). Methods and Materials: The HPLF were exposed to nicotine concentrations of 125 to 1500 ėg/ml for 24 hours (h). IL-6 release and cell growth were determined after exposure to the different nicotine concentrations after 24 hours. IL-6 levels were measured by enzyme-linked immunosorbent assay (ELISA). Cell counts were determined with a Coulter counter. Results: HPLF cell counts were significantly higher than untreated cells after nicotine exposure of 375 ėg/ml and significantly lower than untreated cells for cells treated with 1500 ėg/ml nicotine. After 24 h of cell exposure to nicotine concentrations of 250 and 375 ėg/ml, HPLF release of IL-6 per cell was significantly higher than that of untreated HPLF. HPLF treated with 1500 ėg/ml, nicotine released significantly less IL-6 per cell than did untreated cells. Conclusions: These results indicate that nicotine can increase IL-6 release from HPLF.


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