Home Subscription Services
 
   

 
Oral Biosciences & Medicine
OBM Home Page
About the Editor
Editorial Board
Submit
Author Guidelines
Submission Form
Reprints / Articles
Permissions
Advertising
MEDLINE Search
 
 
 
 
 
FacebookTwitterYouTube
Quintessence Publishing: Journals: OBM

 

Oral Biosciences & Medicine

Edited by Birgitte Nauntofte, Jesper Reibel. Peter A. Reichart, Jim Sciubba, and Joanna M. Zakrzewska

Official publication of the European Society for Oral Laser Applications

ISSN 1742-3287

Publication:
Oral Biosciences & Medicine

Year 2004
Volume 1 , Issue 2

Back
Pages: 123 - 133

Local Cellular Immune Reaction to Oral Normal and Immunodeficient Mice: Implications of Infectious Dose

Andrea Maria Schmidt-Westhausen

Purpose:
To address two questions by means of an animal model: Firstly, does a dose/effect relationship exist between C. albicans load and the emergence of an oral C. albicans infection; secondly, which cellular immune response takes place following inoculation with defined pathogen loads.

Material and Methods:
Immunocompetent inbred mice (Balb/c) (n=19) and mice with combined B- and T-cell defects (SCID) (n=21) were orally inoculated with pathogen loads between 104 and 108C. albicans cells/10 l (strain ATCC 32032). One half of the tongue tissue was examined with the Periodic Acid Schiff (PAS) method for displaying the invasion of hyphae. The other half of the tissue was examined by immune peroxidase technique for analyzing the distribution of immunocompetent cells (CD4, CD54, CD74, CD103) in the epithelial layers and subepithelial connective tissue.

Results:
One week following the inoculation, neither groups tissue showed clinical signs of oral candidiasis. Using PAS preparation hyphae were seen with the inoculation dose of 108C. albicans cells in the case of Balb/c mice and a load of 105 pathogens in SCID mice. The extent of the immunologic reaction depended both on the inoculation dose given to the animals and on their immune status.

Conclusion:
Despite the lack of clinical signs of oral candidiasis in both groups tissue, infections of the tongue epithelium were evident histologically leading to immunologic reactions of the tongue mucosa.

Key words:
Candida, candidiasis, immunologic dose response relationship, oral

 

  © 2014 Quintessence Publishing Co Inc
 

Home | Subscription Services | Books | Journals | Multimedia | Events | Blog
Terms of Use | Privacy Policy | About Us | Contact Us | Advertising | Help | Sitemap | Catalog