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Journal of Oral Laser Applications

Year 2003
Volume 3 , Issue 2

Pages: 87 - 91

Action of the Nd:YAG Laser in Lactobacillus Culture: An In Vitro Study

Maria Margarete Rodrigues Palomares Jacobs/Edmir Matson/Carlos de Paula Eduardo/Margareth Oda

Purpose: The antimicrobial activity of the Nd:YAG laser was evaluated in vitro on the cariogenic oral bacterial Lactobacillus sp. Materials and Methods: Various power settings and times of exposure were used at a fixed distance of 3 cm. The cultures of Lactobacillus sp were irradiated, sowed, and incubated, and bacterial conlonies in the irradiated group were counted. Nonirradiated controls were analyzed following the same procedure. Results: Bacterial inhibition was found in all lased groups. Energy of 40 mJ per pulse and 20 Hz applied from a distance of 3 cm for 4 min was enough to obtain a reduction rate of 96.58% of the Lactobacillus sp. Conclusion: The bactericidal efficacy reported here is comparable to the results of other in vitro studies. Irradiation of Lactobacillus sp with the Nd:YAG laser offers the promise of future implementation in preventive dentistry.


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