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Journal of Oral Laser Applications

Year 2003
Volume 3 , Issue 2

Pages: 79 - 81

The Effect of Er:YAG Laser Treatment on Dentin Collagen: An SEM Investigation

Paolo Benazzato/Alessandro Stefani

Purpose: To evaluate the SEM morphology of dentin collagen fibers after dentin preparation with Er:YAG laser followed by conventional acid etching. Materials and Methods: From 10 freshly extracted human third molars, 10 disks were made from the occlusal portion of dentin. Surfaces were treated with Er:YAG laser (2940 nm) and cooled with water spray. The laser was set at 5.1 W (170 mJ, 30 Hz) using a 600-m-diameter contact sapphire tip moving 1 to 2 mm from the tissue. Specimens were etched with H3PO4 37% acid gel (3M) for 30 and 60 s. After rinsing, collagen fibers were fixed with glutaric aldehyde 2.5% in sodium cacodylate buffer for 12 h at 4C. After dehydration with ethanol, specimens were dried with hexamethyl disilazane and then observed through SEM. Results: After laser treatment and acid etching, collagen fibers seemed to be fused and denatured, but higher magnification revealed a normal appearance into the first part of tubules where thermal increase is lower. There was no difference between groups etched for 30 and 60 s. Conclusion: Er:YAG laser dentin ablation plus air-water spray denatures dentinal collagen fibers. In the more superficial region of dentinal tubules, normal collagen fibers are found, meaning resin bonding agents may be able to produce a hybrid layer in this portion.


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