The purpose of the present study was to examine the effectiveness of Nd:YAG laser in the removal of abnormal (prominent) frenum or frenectomy and evaluate the clinical outcome. Materials and Methods: A total of 10 patients with a prominent frenum between the two central maxillary incisors was selected for this study. A horizontal incision/ablation was made by using continuous wave mode of the pulsed Nd:YAG laser in a contact mode at 200 mJ pulse energy at 5 W output power with 20 Hz (55.6 J/cm²). Each patient was examined during the therapeutical procedure and postoperatively at 3, 7, and 15 days. Results: No adverse reaction, eg, systemic/localized allergy, was observed in any of the cases. Cutting efficiency was excellent with the laser device only; neither carbonization nor scarring of the operation site was noted. At 7 to 15 days following completion of the therapy, the treated area was healed. There was no postoperative pain or swelling. Conclusion: Pulsed Nd:YAG laser therapy is considered a safe and effective method for frenectomy in the clinic, especially if carried out with the appropriate parameters and techniques.