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Quintessence Publishing: Journals: JOLA


The Journal of Oral Laser Applications

Edited by Prof Dr Andreas Moritz and Prof G. Lynn Powell

Official publication of the European Society for Oral Laser Applications

ISSN (print) 1473-7809 • ISSN (online) 1867-5611

Fall 2005
Volume 5 , Issue 3

Pages: 145-150
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Effects of Low-level Hard Laser Irradiation on Mineralization in Three-dimensional Cultured Rat Bone Marrow Cells

Gomi, Kazuhiro/Yashima, Akihiro/Shirakawa, Satoshi/Kanazashi, Mikimoto/Kobayashi, Kazuyuki/Nagano, Takatoshi/Yamagichi, Hiroyasu/Arai, Takashi

Purpose: It is known that the low-power irradiation of a high-output laser accelerates wound healing. However, there is little fundamental research on it, especially for cultured osteoblasts. The purpose of this study was to evaluate the effect of low-level irradiation of a hard laser on cultured osteoblasts. Materials and Methods: Osteoblasts cultured in collagen gel were irradiated using either a CO2 or a semiconductor laser. Expressions of OC, BSP, and HSP27 were examined at 3 days after irradiation and calcium volumes were measured after 1 week. Results: In the CO2 laser group, a significant difference was only observed with 2.6-W laser irradiation. There was no promoting effect of semiconductor laser irradiation. Additionally, the highest expression of HSP27 was shown with 2.6 W of CO2 laser irradiation. Conclusion: The CO2 laser at 2.6 W can increase the calcification of osteoblasts in three-dimensional cultures. The mechanism seems to be related to the cascade of HSP27.

Keywords: carbon dioxide laser, semiconductor laser, calcification, heat shock protein 27, osteoblast, low-level irradiation

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