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Quintessence Publishing: Journals: JOLA

 

The Journal of Oral Laser Applications

Edited by Prof Dr Andreas Moritz and Prof G. Lynn Powell

Official publication of the European Society for Oral Laser Applications

ISSN (print) 1473-7809 • ISSN (online) 1867-5611

Publication:
JOLA
Fall 2006
Volume 6 , Issue 3

Pages: 169-180
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Immunohistochemical and Electron Microscopic Observations of Rat Palatal Mucosa after CO2 Laser Irradiation

Koide, Naohiro / Murakami, Satoshi / Tokikawa, Akihiro / Matsuzaka, Kenichi / Inoue, Takashi

Purpose: The purpose of this study was to investigate the morphological changes of rat palatal mucosa after CO2 laser irradiation. Materials and Methods: The laser ablations were made with the CO2 laser in continuous wave mode, 1 W, for 5 s. For light microscopic observations, animals were killed immediately, 0.5, 1 to 5 and 7 days after irradiation. The sections were stained immunohistochemically with primary antibodies: laminin, S100, polyclonal-keratin, HMB45 and CK20. For electron microscopic observation, tissues were fixed immediately and 12 h after irradiation. Results: Immediately after irradiation, degeneration of the superficial layer of the epithelium was observed; however, the cells of the basal and para-basal layers were well preserved. Twelve hours after irradiation, the epithelium had degenerated; however, a laminin-positive reaction was still recognized at the basal membrane. The electron microscopy showed that, although the cell membranes had become unclear, desmosome-like structures were preserved. One day after the laser irradiation, the epithelium had separated from underneath the lamina propria. At 2 days after the irradiation, the irradiated areas were covered by fibrin materials without any visible damage of the connective tissue. At 7 days after the irradiation, the epithelium had regenerated completely without any cells positive for S-100, HMB45 or CK20. Conclusion: These results suggest that the CO2 laser affected binding proteins of the basal membrane without destroying the underlying connective tissue.

Keywords: CO2 laser, oral mucosa, immunohistochemistry, electron microscopy

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