Purpose: To determine the influence of aging in artificial saliva compared to distilled water on the dentin microtensile
bond strength (μTBS) of different adhesive systems.
Materials and Methods: Occlusal enamel and superficial dentin of 42 teeth were removed and roots were sectioned
to expose the pulp chamber for connecting the tooth segments to an intrapulpal pressure assembly. According
to the tested adhesives, tooth segments were allocated to three groups (n = 14): an etch-and-rinse adhesive
(Adper Scotchbond Multi-Purpose, SBMP), a two-step self-etching adhesive (Clearfil SE Bond, CSE), and a singlestep
self-etching adhesive (Clearfil S3 Bond, S3). Each adhesive system was applied to the dentin surface according
to its manufacturers instructions, while intrapulpal pressure was simulated. Resin composite (3M ESPE) was
built up in two increments of 2 mm each. Each bonded specimen was sectioned to obtain eight sticks
(0.8 ± 0.01 mm2). Sticks of each group were divided equally (n = 56) according to the storage solution, either distilled
water or artificial saliva. For each storage solution, half of the sticks of each subgroup (n = 28) was stored
for 24 h at 37ĘC and the other half was thermocycled for 10,000 cycles between 5ĘC and 55ĘC. Sticks were then
subjected to μTBS testing. Data were statistically analyzed using multifactor ANOVA with repeated measures and
Bonferronis post-hoc test (p < 0.05). Students t-test was used for pairwise comparison. Failure modes were determined for all tested sticks using scanning electron microscopy.
Results: The decrease in bond strength of the three adhesives was significantly higher in distilled water than in artificial
saliva. The predominant failure modes were adhesive and mixed.
Conclusion: The decrease in bond strength was more pronounced for specimens stored in distilled water than in