Share Page:

Volume 12 , Issue 1
January/February 1999

Pages 83-86

Adherence of Candida albicans to the Surface of Polymethylmethacrylate-E Glass Fiber Composite Used in Dentures


PMID: 10196833

Purpose: The use of reinforcing fibers in dentures has raised concerns about possible increased adherence of yeasts t o the surface. The aim of this in vitro study was to compare the adherence of Candida albicans to the surface of denture-base polymer and to E-glass fibers. Materials and Methods: Test specimens were made from an autopolymerized denture-base resin (Palapress) reinforced with preimpregnated unidirectional E-glass fibers, which were exposed at the surface. The test specimens were pretreated with parotid saliva and incubated without agitation in standardized yeast suspensions (10 to the eighth power colony-forming units per mL) in phosphate-buffered saline at 37 degrees C for 1 hour. The test specimens were then washed to remove nonadherent cells. After being air dried, they were sputter coated with gold-palladium for scanning electron microscopy (SEM). To compare the adherence to different surfaces, the num ber of yeast cells found either on the polymer matrix or on the glass fibers was counted from SEM fields (170 um x 120 um, 600x) of randomly selected areas. Results: The mean density of yeast cells found on the surface of the polymer matrix was significantly higher (P < 0.001) than that on the surface of glass fibers. The number of adherent yeast cells found at the interface between the fibers and polymer matrix was high. Conclusion: The adherence of C albicans to E-glass fibers was lower than to polymer matrix in the denture composite. If fibers are exposed only during polishing of the composite, the reinforcing material appears not to increase the adherence of this common oral yeast. However, areas with permanently exposed fibers may provide mechanical retention for yeast cells at the interface of the components.

Full Text PDF File | Order Article


Get Adobe Reader
Adobe Acrobat Reader is required to view PDF files. This is a free program available from the Adobe web site.
Follow the download directions on the Adobe web site to get your copy of Adobe Acrobat Reader.


© 2017 Quintessence Publishing Co, Inc

IJP Home
Current Issue
Ahead of Print
Author Guidelines
Accepted Manuscripts
Submission Form
Quintessence Home
Terms of Use
Privacy Policy
About Us
Contact Us