Home Subscription Services

The International Journal of Prosthodontics
IJP Home Page
About the Editor
Editorial Board
Accepted Manuscripts
Author Guidelines
Submission Form
Reprints / Articles
Quintessence Publishing: Journals: IJP
The International Journal of Prosthodontics

Edited by George A. Zarb, BChD, DDS, MS, MS, FRCD(C)

ISSN 0893-2174

September/October 2001
Volume 14 , Issue 5

Share Abstract:

Adherence of CandidaSpecies to Newly Polymerized and Water-Stored Denture Base Polymers

Tuomas Waltimo, DDS, PhD, Pekka Vallittu, DDS, PhD, CDT, Markus Haapasalo, DDS, PhD

PMID: 12066642

Purpose: The aim of this in vitro study was to examine the adherence of yeasts to newly polymerized and water-stored denture base polymers using four Candidastrains with different cell surface hydrophobicities. Materials and Methods: Thirty-two autopolymerized denture base polymer specimens were stored in distilled water at 37C for 7 days and 32 were newly polymerized. Sixteen specimens of each group were pretreated with unstimulated mixed saliva (saliva group), and 16 were pretreated with phosphate-buffered saline ([PBS] PBS group) at 37C for 1 hour. Hydrophobicity of the newly polymerized and water-stored specimen surfaces was determined by contact angle measurements. C guilliermondii, C albicans, C glabrata,and C tropicaliswere grown on tryptic soy agar. They were pretreated either with saliva or PBS and suspended in PBS. Four parallel test specimens were incubated without agitation in each yeast suspension at 37C for 1 hour, washed, and allowed to dry in air. Adherent cells were fixed, gram stained, and counted from 10 high-power light-microscopy fields of each specimen. Results: Enhanced adherence of yeasts was observed in water-stored test specimens in comparison to newly polymerized test specimens. Salivary pellicle reduced the hydrophobicity of test specimens and the adherence of yeasts. No differences in contact angles of distilled water between newly polymerized and water-stored polymers were observed. Conclusion: Yeasts seem to adhere less to newly polymerized than waterstored denture base polymer. This may be due to the release of residual monomer from the newly polymerized material.

Full Text PDF File | Order Article


Get Adobe Reader
Adobe Acrobat Reader is required to view PDF files. This is a free program available from the Adobe web site.
Follow the download directions on the Adobe web site to get your copy of Adobe Acrobat Reader.
  © 2015 Quintessence Publishing Co Inc

Home | Subscription Services | Books | Journals | Multimedia | Events | Blog
Terms of Use | Privacy Policy | About Us | Contact Us | Advertising | Help | Sitemap | Catalog