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Volume 19 , Issue 1
January/February 2006

Pages 1316


In Vitro Assessment of Cytotoxicity of Resin-Based Dental Restorative Materials on WEHI 13 Var Fibroblasts

Athina A. Bakopoulou, DDS / Ioanna N. Triviai, BS / Asterios S. Tsiftsoglou, BS, MS, MPhil, PhD / Paylos D. Garefis, DDS, PhD


PMID: 16479751

Purpose: The aim of this study was to assess the cytotoxicity of 4 resin composites’ eluates on WEHI 13 Var fibroblasts as they aged in a biologic medium. Materials and Methods: Cytotoxicity was determined by counting the number of viable cells by trypan blue exclusion. Morphologic changes attributable to cytotoxicity were observed by May-Grunwald-Giemsa cytologic staining and microscopic examination. DNA gel electrophoresis was performed to detect possible genotoxicity and DNA damage. Results: All resin composite eluates, except that for Targis, caused a pronounced cytotoxicity during the first 72 hours that gradually decreased after 2 weeks of aging. Severe morphologic alterations and pronounced DNA damage were also observed. Conclusion: These findings indicate that resin-based dental restorative materials release agents cytotoxic and genotoxic to fibroblasts. Cytotoxicity is gradually decreased as the composite resins age in a biologic-relevant medium.
Int J Prosthodont 2006;19:1316.


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