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ENDO - Endodontic Practice Today

Edited by Bun San Chong and Edgar Schäfer

Official Publication of the Belgian Association for Endodontology and Traumatology (BAET) and the French Society of Endodontics (SFE)

ISSN (print) 1753-2809 • ISSN (online) 1753-2817


Fall 2019
Volume 13 , Issue 3

Pages: 255–263
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An ex vivo study to determine the antibacterial efficacy of chitosan nanoparticles, calcium hydroxide and double antibiotic paste as intracanal medicaments against Enterococcus faecalis biofilm

Mai M. Hussein, Amr M. Abdallah, Nayera A. Mokhless, Marwa A. Meheissen

Objective: To assess the antibacterial efficacy of chitosan nanoparticles (CSNPs), calcium hydroxide (Ca(OH)2) and double antibiotic paste (DAP) against 3-week-old Enterococcus faecalis biofilm.

Materials and methods: Sixty extracted mandibular premolars were standardised to a specimen length (14 mm), instrumented and, after sterilisation, inoculated with E. faecalis and incubated for 3 weeks. Biofilm formation was confirmed by scanning electron microscopy in four randomly selected specimens. The remaining specimens (n = 56) were divided into four groups (n = 14): Group 1, 20 mg/ml CSNPs in methylcellulose; Group 2, 35% Ca(OH)2 in methylcellulose; Group 3, 1 mg/ml DAP in methylcellulose; and Group 4, methylcellulose gel (control group). Each group was further divided into two equal subgroups (n = 7) and incubated for 7 or 14 days. After each test period, a sample of the biofilm was collected from each specimen, plated, and the antimicrobial efficacy assessed via colony forming units count (CFU/ml).

Results: All the antibacterial agents evaluated resulted in complete eradication of E. faecalis after 14 days. However, Ca(OH)2 was the only medicament that provided a significant bacterial count reduction after 7 days (P < 0.05). No statistically significant difference was found between Ca(OH)2, CSNPs, and DAP (P ≥ 0.05).

Conclusions: A 14-day period of dressing with CSNPs, Ca(OH)2, or DAP resulted in effective antibacterial action on the 3-week-old E. faecalis biofilm.

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