Home Subscription Services

Endodontic Practice Today
ENDO Home Page
About the Editor
Editorial Board
Author Guidelines
Submission Form
Reprints / Articles
Official Site
Quintessence Publishing: Journals: ENDO


ENDO - Endodontic Practice Today

Edited by Antionio Bonaccorso, Bun San Chong, Edgar Schafer, and Luc van der Sluis

Official Publication of the Belgian Association for Endodontology and Traumatology (BAET), the French Society of Endodontics (SFE) and the Norwegian Endodontic Society (NEF)

ISSN (print) 1753-2809 • ISSN (online) 1753-2817


Spring 2014
Volume 8 , Issue 1

Pages: 57-62
Share Abstract:

Evaluation of cytotoxicity and physicochemical properties of silicone-based endodontic sealer GuttaFlow 2

Silva, Emmanuel J.N.L. / Herrera, Daniel R. / Rosa, Tiago P. / Duque, Thais M. / Gomes, Brenda P.F.A. / Zaia, Alexandre A.

Introduction: The aim of the study was to evaluate the cytotoxicity, radiopacity, pH, flow and solubility of a silicone-based and an epoxy resin-based endodontic sealer: GuttaFlow 2 and AH Plus, respectively.
Methods: Cytotoxicity, radiopacity and flow evaluation were performed following ISO requirements. pH was measured at periods of 3, 24, 72, 168 h. Cytotoxicity was evaluated by MTT assay to check the Balb/c 3T3 cells viability at 1 to 3 week periods. Data were statistically analysed by ANOVA and Tukey test with a significance level of 5%.
Results: GuttaFlow 2 was non-cytotoxic in all tested periods. AH Plus was more cytotoxic than GuttaFlow 2 when freshly mixed and after 1 week (P < 0.05). After 2 weeks, AH Plus becomes noncytotoxic. Although AH Plus presented higher radiopacity than GuttaFlow 2 (P < 0.05), both sealers showed minimum required values. GuttaFlow 2 and AH Plus presented a pH level close to neutral in all tested times. AH Plus also showed significantly lower flow than that of GuttaFlow 2 (P < 0.05). Both sealers showed values complying with the solubility minimum requirements.
Conclusion: GuttaFlow 2 demonstrated suitable biological and physicochemical properties for an endodontic sealer.

Keywords: AH Plus, cytotoxicity, GuttaFlow 2, physicochemical properties, root canal sealer

Full Text PDF File | Order Article


  © 2015 Quintessence Publishing Co Inc

Home | Subscription Services | Books | Journals | Multimedia | Events | Blog
Terms of Use | Privacy Policy | About Us | Contact Us | Advertising | Help | Sitemap | Catalog