Home Subscription Services
 
   

 
The Chinese Journal of Dental Research
About the Editor
Editorial Board
Author Guidelines
Submission Form
Reprints / Articles
Permissions
Advertising
MEDLINE Search
Official Site
 
 
 
 
 
FacebookTwitterYouTube

Publication:
The Chinese Journal of Dental Research

Year 2007
Volume 10 , Issue 2

Back
Pages: 33 - 37

Study of BMP-2 Gene Modified Goat Bone Marrow Stromal Cells in Promoting New Bone Formation

JIANG, Xin Quan / ZHANG, Xiu Li / HU, Jing Zhou / ZHANG, Zhi Yuan


Objective: To evaluate the osteoinductive effects of adenoviral encoding human BMP-2 (AdBMP-2) gene modified goat bone marrow stromal cells (bMSCs) in nude mice.
Methods: Primary cultured goat bMSCs were transduced with 50 pfu/ml AdBMP-2 and Ad- LacZ. Gene transfer efficiency was detected by X-gal staining 3 days after AdLacZ gene transduction. AdBMP-2 or AdLacZ gene modified bMSCs were injected into nude mice intramuscularly (number of injection sites: AdBMP-2 = 9; AdLacZ = 9). Three samples in each group were obtained after 2 weeks, and the remaining six were harvested after 4 weeks. All samples were evaluated by histology. MicroCT, BMP-2 immunohistochemistry and X-gal staining were performed for 4-week samples.
Results: Gene transfer efficiency reached above 70%. Two weeks after injection into nude mice. AdBMP-2 samples demonstrated a combination of relatively mature bone formation and a lesser amount of cartilaginous tissue. In contrast, only fibroblastic-like tissue was detected in Ad- LacZ-transduced bMSCs samples. At week 4, all six AdBMP-2 sites had new bone formation, while in the AdLacZ group no obvious radiopaque area was detectable by microCT. Histologically, AdBMP-2 transduction induced a large bone mass with fatty marrow tissue. While the AdLacZ group showed mainly fibrotic tissue proliferation, X-gal staining confirmed the bMSCs origin in the AdLacZ group.
Conclusion: bMSCs modified with AdBMP-2 gene can promote new bone formation in a nude mice model and could be used for further bone regeneration in the oral and maxillofacial region.

Keywords: bone marrow stromal cells, bone morphogenetic protein, gene therapy

 

 

  © 2014 Quintessence Publishing Co Inc
 

Home | Subscription Services | Books | Journals | Multimedia | Events | Blog
Terms of Use | Privacy Policy | About Us | Contact Us | Advertising | Help | Sitemap | Catalog