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The Chinese Journal of Dental Research

Year 2004
Volume 7 , Issue 3

Pages: 22 - 28

Simvastatin May Inhibit Osteoclast-Like Cell Formation by Decreasing the Ratio of RANK/OPG mRNA Expression in Bone Marrow Stromal Cells In Vitro

Liu Bo/Yu Shi Feng

Objective: Simvastatin, an inhibitor of 3-hydroxy-3-gulutaryl-coenzyme A (HMG-CoA) reductase, may enhance bone formation by promoting the expression and synthesis of bone morphogenetic protein-2 (BMP-2) of osteoblasts. The purpose of this study is to elucidate its effect to osteoclasts. Methods: Osteoclast-like multinucleated cells were induced by bone marrow cells and osteoblasts coculture system in the presence of 1,25(OH)2VitD3. The numbers of tartrage-resistant acid phosphatase (TRAP)-positive multinucleated cells and mononuclear cells and the resorption lacuna were analyzed after treatment with Simvastatin. The expression of RANKL and OPG mRNA were also evaluated by RT-PCR with bone marrow stromal cells after treatment with Simvastatin for 24 hours. Results: The numbers of TRAP-positive osteoclast-like multinucleated cells and mononuclear cells and the bone resorption activities were reduced by a dose-dependent manner after treatment with Simvastatin. The ratio of RANKL/OPG mRNA was also decreased after application of Simvastatin for 24 hours in bone marrow stromal cells. Conclusion: Simvastatin may suppress osteoclast-like cell differentiation and formation in vitro. The mechanism may be due to the decreasing expression ratio of RANKL/OPG mRNA by bone marrow stromal cells.



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