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The Chinese Journal of Dental Research

Year 2004
Volume 7 , Issue 1

Pages: 45 - 48

Detection of Methicillin-Resistant Staphylococcus Species in the Dental Plaque of Disabled Patients

Rongsend Liu, DDS, MSc/Ma Liang, DDS/Shufeng Han, DDS/Qingyu Yang, DDS/Hongchen Liu, DDs, MSc, PhD

Objective: To investigate the carriage rate of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase-negative staphylococcus (MR-CNS) in the dental plaque of patients with disabilities. Methods: A multiplex polymerase chain reaction (PCR) method of coamplification of femA and mecA determinants was used for detection of MRSA and MR-CNS in the dental plaques of 200 disabled patients. Results: The carriage rate of MRSA and MR-CNS was 3% and 14.5%, respectively. The phenotypes of the MR-CNS were MR-S epidermidis and MR-S haemolyticus. Conclusions: MRSA and MR-CNS oral carriers are not unusual among disabled patients attending dental clinics, and the multiplex-PCR technique is a specific and rapid alternative method for detection of MRSA and MR-CNS.



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