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Publication:
The Chinese Journal of Dental Research

Year 2002
Volume 5 , Issue 2

Back
Pages: 12 - 17

The Expression Level of AnnexinI and cPLA2 in Dexamethasone-Induced Cleft-palate Mice

Bing Shi, DDS, MS, PhD/Hui Zuo, DDS, MS, PhD/Zuyi Mao, MS/Yu Lu, MS

Objective: To test the changing expression level of AnnexinI and cPLA2 in the palatine process of cleft-palate mice A/J and C57B/6j which are induced by dexamethasone, and to discuss the developing mechanism of cleft palate and the corresponding preventive methods. Methods: Pregnant mice A/J and C57B/6j were randomly divided into a normal untreated control group, a group with deformities induced by dexamethasone, a group also given VitB12 as an antidote to the deforming factor, and a normal group given only VitB12. The relative quantities of AnnexinI and cPLA2 were tested by immunoblotting. Results: Among mice A/J, with the development of the palatine process, the expression level of AnnexinI was increased in the normal group, and other groups showed a similar change. The expression level of AnnexinI was significantly higher in group DEX and group DEX + VitB12 than in the normal group and the VitB12 group at any one time, while there was no significant difference between the normal group and the VitB12 group. However, the change in the expression level of cPLA2 was in the opposite direction: with development it decreased in the palatine processes of normal mice. In mice C57B/6j there was no significant difference between the normal group and group DEX in the measured quantity of AnnexinI and cPLA2. Conclusion: AnnexinI and cPLA2 introduce glucocorticoid which induces cleft palate. VitB12 cannot inhibit the enhancing effect of DEX on the expression level of AnnexinI, but it can prevent the inhibiting effect of DEX on the expression level of cPLA2, which is probably one of the reasons why VitB12 prevents the deforming effects of glucocorticoid.

 

 

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