Objective: The ideal denture adhesive demonstrates a minimal toxicity to the mucosa. The purpose of this study was to evaluate the in vitro cytotoxicity of a new denture adhesive. Material and Methods: Samples were prepared and extracted in Dulbecco’s Modified Eagle’s Medium. The cell viability of human oral fibroblast cells cultivated for 24 hours with DA-containing medium was evaluated by the MTT assay to investigate cell proliferation. Optical densities were measured by a spectrophotometer at 490 nm. Results: The statistical analysis of results showed that this denture adhesive displayed only slight cytotoxicity. Conclusion: The generally favorable in vitro cytotoxicity of the denture adhesive formulation indicates that this product may be efficacious.