Objective: To investigate the effect of Nifedipine, a calcium channel blocker, on cellular proliferation in gingival fibroblasts in vitro. Methods: Gingival fibroblasts were cultured and incubated with various concentrations of Nifedipine (23.5, 108, 360, and 1200 ng/mL) for up to 7 days. Results: Cell numbers were significantly different among these groups on the sixth and seventh days, especially between the 1200 ng/mL or 360 ng/mL and the other concentrations. Conclusion: Nifedipine can influence proliferation of gingival fibroblasts in high concentrations.