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The Chinese Journal of Dental Research

Year 2000
Volume 3 , Issue 4

Pages: 35 - 39

Alteration of Fas/FasL Expression of Oral squamous Cell Carcinoma Cell Line Tca83 Induced by Retinoic Acid

Wei Qun Guan, DDS, PhD/Shi Fen Yu, DDS, MS/Yan Gao, DDS, PhD

Objective: To elucidate the chemosensitivity of human tongue squamous cell carcinoma Tca83 cell line to all-trans retinoid acid (RA) and to determine the potential genetic factors mediating this sensitivity. Methods: RA in the amounts of 0.1, 1, and 10 ÁMol/L were used to treat human cell line Tca83 in vitro. Cell apoptosis as well as Fas/FasL expression were analyzed by TUNEL, RT-PCR, and immunohistochemistry approaches. Results: RA in the amounts of 1 and 10 ÁMol/L RA began to inhibit cell proliferation at day 3 and had induced cell apoptosis on day 5. Both Fas and FasL could be detected in the treated and untreated cells. RA upregulated Fas mRNA and protein expression at day 5. RA had no obvious effect on FasL expression. Conclusion: The results suggest that apoptosis induced by RA may result from the enhancement of Fas gene transcription and translation.



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