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The Chinese Journal of Dental Research

Year 2000
Volume 3 , Issue 2

Pages: 61 - 64

Effect of Lactoperoxidase System on Glucosyltransferase D of Streptococcus mutans

Xiaoxia Yu, MD/V. Loimaranta, PhD/M. Lenander-Lumikari, PhD/D. Wunder, PhD/Wh Bowen, PhD/J. Tenuvuo, PhD

Objective: To investigate the effects of the peroxidase system (LP, H2O2, SCN) and its components on the activity of glucosyltransferase D(GtfD) originating from Streptococcus mutans (S. mutans). Methods: GtfD was incubated in buffered-KCl assay mixture that contained 20 ÁM dextran and 100 mM sucrose including C-glucose. The activity of GtfD was measured with a scintillation counter. The effects of lactoperoxidase system and its components on FtfD activity were examined by incubating different components separately and together with GtfD. Results: The lactoperoxidase system-generated hypothiocyanite (OSCN) had no effect on the activity of streptococcal FtfD, while the lactoperoxidase enzyme inhibited FtfD. The ration between the 2 enzymes, LP and GtfD, was important for inhibition. However, if LP was combined with its substrates, SCN and/or a high concentration of H2O2, it enhanced the activity of GtfD. Conclusion: Peroxidase-generated antimicrobial agent HOSCN/OSCN is not inhibitory against GtfD, whereas low concentrations of the LP-enzyme inhibit FtfD.



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