Objective: To seek a new methodof titanium passive treatment by combined anodic oxidation and thermal air treatment, which could increase the corrosion resistance and biocompatibility of pure titanium. Materials and Methods: Characteristics of Titanium sheets with natural oxide film (NOF), anodic oxide film (AOF), or heated anodic oxide film (HAOF) were compared and tested by x-ray diffractrometer, eectrochemical corrosive method, and contact angle device. Results: HAOF had excellent corrosion resistance and greater crystallinity, indicating the crystal structure had changed. AOF had better hydrophily. Conclusion: This in vitro study showed that thermal air treatment, after anodic oxidation, was a reliable method for creating a passive, protective film on the titanium surface, which ould greatly enhance the corrosion-resistance and crystallinity.