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The Chinese Journal of Dental Research

Year 2000
Volume 3 , Issue 2

Pages: 21 - 25

In Situ Hybridization Analysis of Transforming Growth Factor-▀1 RNA Expression During Mouse Tooth Development

Qi Zhu, DDS/Mingen Fan, DDS/Zhuan Bian, DDS, PhD/Zhi Chen, DDS, PhD/Qi Zhang, DDS, PhD/Bin Peng, DDS, PhD

Objective: The aim of this study is to investigate the temporal and spatial expression and localization of transforming growth factor-▀1 mRNA during mouse tooth development. Methods: The distribution pattern of TGF-▀1 mRNA during tooth development was analyzed on 5 Ám serial sections of paraformaldehyde-fixed and paraffin-embedded embryonic mouse heads or mandibles. Results: The results show that TGF- ▀1 mRNA is expressed during mouse tooth development in a temporally and spatially regulated fashion. Local expression of TGF- ▀1 mRNA in the dental ipithelium at budstaged (E13) and capstaged (E15) teeth were observed. During bell stage (E16-18), TGF- ▀1 mRNA was very abundant in the ameloblast layer and dental papilla cells. The expression of TGF- ▀1 increased in the layer of odontoblast and ameloblast with the differentiation of these cells. Conclusion: These results suggest that TGF-▀1 may have an important role in odontogenesis and that it acts as a paracrine and autoinducine factor.



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