Objective: To evaluate the particular expression of Shh and its receptor patched (Ptch) involved in mouse embryonic craniofacial development. Methods: The expression patterns of Shh pathway genes during murine embryonic craniofacial development were investigated by applying in-situ hybridization studies of whole-mount and sections, immunohistochemistry, and reverse transcription polymerase chain reaction analysis. Results: Shh was expressed in the mouse embryo at 11 and 12.5 days postcoitum (dpc); Ptch was expressed at 11, 12.5, and 14.5 dpc, but expression patterns were different. SHH protein could also be detected at 11, 12.5, and 14.5 dpc, confirming the gene expression studies. Conclusion: Shh and Ptch expression patterns are characterized. The data suggest that Shh and Ptch are involved during craniofacial development and their roles may vary.
Keywords: immunohistochemistry, in-situ hybridization (ISH), maxillofacial region development, Shh, Ptch