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The Chinese Journal of Dental Research

Edited by Xu Chen MA

Official journal of the Chinese Stomatological Association

ISSN (print) 1462-6446 • ISSN (online) 1867-5646

Publication:

Spring 2010
Volume 13 , Issue 1

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Involvement of Notch Signalling Pathway in Senescence of Human Dental Pulp Cells

ZOU, Xiao Ying / ZHUANG, Heng / YUE, Lin / GAO, Xue Jun

Pages: 45 - 49

Objective: To evaluate whether the Notch signalling pathway is involved in the senescence of human dental pulp cells. Methods: Human dental pulp cells were isolated and cultured. The Notch signalling pathway was blocked by adding DAPT (N-[N-(3,5-difluorophenacetyl-L-alanyl)]-S-phenylglycine t-butyl ester, γ-secretase inhibitor, 5 μmol/L) into the culture medium. Cell proliferation was evaluated by MTT assay. Cell senescence was evaluated through expression of senescenceassociated β-galactosidase (SA-β-Gal) using β-galactosidase staining. Results: The proliferation of the human dental pulp cells decreased dramatically after treatment with DAPT, compared with the control. SA-β-Gal positive staining cells were seen in the DAPT group, but not in the control group. Conclusion: The Notch signalling pathway is involved in the senescence of human dental pulp cells.

Keywords: dental pulp cells, senescence, Notch signalling pathway

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