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Quintessence Publishing: Journals: CJDR

 

The Chinese Journal of Dental Research

Edited by Xu Chen MA

Official journal of the Chinese Stomatological Association

ISSN (print) 1462-6446 • ISSN (online) 1867-5646

Publication:

Winter 2012
Volume 15 , Issue 2

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Nano-sized 58S Bioactive Glass Enhances Proliferation and Osteogenic Genes Expression of Osteoblast-like Cells

Gong, Wei Yu / Dong, Yan Mei / Chen, Xiao Feng / Karabucak, Bekir

Pages: 145 - 152

Objective: To observe the effects of ionic dissolution products on nano-sized 58S bioactive glass (nano-58S) on proliferation and specific osteogenic genes expression in MG-63 cells.
Methods: Ionic dissolution products were prepared by incubating nano-58S or sol-gel bioactive glass 58S (58S) particulates in Dulbecco’s modified Eagle’s medium (DMEM) at 1% w/v for 24 hr and filtrated through 0.22 Ám filters to remove the particulates. MG-63 cells were cultured in the nano-58S extraction, 58S extraction, and DMEM, respectively, for different time periods to assay the proliferation, mRNA expression of alkaline phosphatase (ALP), Cbfa1, Collagen type I (Col-I) and osteocalcin (OCN), as well as ALP staining, activity and matrix mineralisation.
Results: In the nano-58S group, cell proliferation and mRNA expression of ALP, Cbfa1 and OCN were significantly enhanced in a time-dependent manner compared with the control group. mRNA expression of Cbfa1 on day 4 and OCN on day 7 was significantly higher than that in the 58S group. Moreover, there was significantly more ALP protein expression and mineralisation in the nano-58S group than in the 58S group.
Conclusion: The nano-58S enhanced proliferation, osteogenic markers expression in MG-63 cells and induced stronger mineralization than 58S.

Keywords: human osteoblast-like cells, nano-sized bioactive glass, gene expression, bone, osteogenesis

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