This lab manual of color oral histology photographs is designed to aid dental and dental hygiene students in identifying the salient features of microscopic anatomy of oral tissues. It presents large, clear, identifiable photos of normal oral tissues and developing teeth accompanied by brief descriptions highlighting the special features of each.
128 pp; 215 color illus;
ISBN: 0-86715-386-5; 0867153865;
Preface:
This atlas is not intended to be an oral histology text; many fine textbooks of oral histology are already available. However, when students enter the laboratory to identify the salient features of microscopic anatomy of oral tissues described in these texts, they find that most of them are woefully deficient in color photographs to aid them in this task. There is a particular need for a photographic atlas of normal oral histology designed as a laboratory aid. It is the purpose of this atlas to meet that need.
To that end, this book contains figures designed to make it easier for students to orient themselves and to locate and identify features of each tissue, as well as a minimum of descriptive text. Most tissues were photographed at low power for orientation and then at increasingly higher magnifications. Magnifications range from Ą150 to Ą640. No electron micrographs are included, since most textbooks provide electron micrographs where appropriate and most microscope laboratories do not offer students the use of an electron microscope. Some commonly used special stains, such as periodic acid–Schiff and Ungewitter nerve stains, have been used, but most of the tissues are stained with hematoxylin and eosin. Glycomethacrylate microslide preparations of many tissues are now available commercially, and many student slide collections may contain a mixture of paraffin- and methacrylate-embedded tissues; both types of preparations are presented here. The glycomethacrylate-embedded tissues are stained with a modified H and E stain, H and Lee (see Laboratory Medicine 1985;16:538–540). Paraffin-embedded sections are from 3- to 8-µm thick, while methacrylate-embedded sections are 1- to 2-µm thick. Undecalcified ground sections of teeth are approximately 130-µm thick. Finally, although most tissues are human, tissues from rhesus monkeys and from pigs are also included.
It is the author’s hope that this work will serve as a valuable laboratory aid for students attempting to recognize the microscopic features of normal oral tissues and as a supplement to the many fine oral histology textbooks currently in use.
No project like this is completed without the help of many people. I would like to acknowledge the many students who recognized the need for such a project and offered encouragement and suggestions along the way. Photographic services provided by the staff of Wolf Camera and by Zeiss microscope technicians and photographic consultants were exceptional. Thanks to Dr Karla Kent for the fluorescence microscope photograph of a slide of a tetracycline-banded tooth. The methacrylate-embedded material was prepared by Nickolas Roman, whose skill and advice were matchless. Special thanks to CharEll Melfi and Christine Moleski, without whom this project would not have been completed; their graphics expertise, patience, persistence, and encouragement were invaluable.
Table of Contents:
1. Tooth Development
2. Enamel
3. Dentin
4. Pulp
5. Cementum
6. Periodontal Ligament and Dentogingival Junction
7. Alveolar Bone
8. Tooth Eruption and Shedding
9. Mucosa
10. Salivary Glands
11. Temporomandibular Join